At this stage sample is washed with water and then edc is recovered step wise in atm and vaccum as described in procedure. so there is possibility of water carry over during contineous operation. We have checked in lab for water content and find 1.3%w/w but it may varry from 1% to 2-3%.For trial as such condition we have to make homogeneous mixture to avoid the layer seperation initially before the trial. 