[three_fourth padding="0 15px 0 0"]<h4>IntegenX Apollo 324 Automated NGS Library Prep</h4>
<ul>
	<li>DNA</li>
	<li>mRNA</li>
	<li>Total RNA</li>
</ul>
Sample Requirements: &gt;50ng in 15ul

<h4>Illumina Nextera</h4>
Nextera is a fast library prep method that requires only a small amount of DNA -as little as 1ng of input. This method uses a transposase to simultaneously fragment and tag DNA with Illumina adapters ("Tagmentation) followed by a limited cycle PCR reaction to incorporate sequencing primers and indices.

Nextera: 50ng input DNA -larger yield, 5 PCR cycles
NexteraXT: 1ng input DNA -smaller yield (ideal for multiplexing), 12 PCR cycles.

<h4>Targeted Resequencing</h4>
<ul>
<li>MBCF Custom Amplicon</li>
<li>Qiagen GeneRead</li>
<li>Illumina Amplicon Panel/Custom</li>
<li>Wafergen</li>
</ul>
Yup, there are a lot of different ways to approach this application.[/three_fourth][one_fourth_last]
<div class="genomics">
<h5>Place an Order</h5>
Login to iLab
<h5>Sample Drop-Off</h5>
We collect our samples from the following locations:
- The Fish Room (near the 3rd floor elevator lobby of the Smith Building)
- CLS Building (11th floor, near the Spiegelman Lab)
- <em>For RNA</em>: Smith Building, 1129 (-80C)
<h5>Get Data</h5>
<a href="http://mbcftp.dfci.harvard.edu/Login" target="_blank">MBCFTP</a>
<h5>Ask a Question</h5>
Send an <a href="mailto:zherbert@mail.dfci.harvard.edu" target="_blank">email to Zach</a></div>[/one_fourth_last]