<b>IntegenX Apollo 324 Automated NGS Library Prep</b>
<ul>
	<li>DNA</li>
	<li>mRNA</li>
	<li>Total RNA</li>
</ul>
Sample Requirements: &gt;50ng in 15ul

<b>Illumina Nextera</b>
Nextera is a fast library prep method that requires only a small amount of DNA -as little as 1ng of input. This method uses a transposase to simultaneously fragment and tag DNA with Illumina adapters ("Tagmentation) followed by a limited cycle PCR reaction to incorporate sequencing primers and indices.

Nextera: 50ng input DNA -larger yield, 5 PCR cycles
NexteraXT: 1ng input DNA -smaller yield (ideal for multiplexing), 12 PCR cycles.

<b>Targeted Resequencing</b>
<ul>
<li>MBCF Custom Amplicon</li>
<li>Qiagen GeneRead</li>
<li>Illumina Amplicon Panel/Custom</li>

Login to iLabs