[three_fourth padding="0 15px 0 0"]<h6>Description</h6>
<h4>Tech Specs</h4>
<h4>Sample Requirements</h4>
<strong>Intact Mass Analysis</strong>
<em>Concentration</em>: Optimally, 10uM. Samples at 1uM work if they are clean and very low buffer and salt content. 
 
<em>Buffer</em>:  Samples presented in water or other volatile solvents are best.  If your sample is not compatible with those requirements, then use a low salt buffer (< 50mM).  Try to avoid phosphate buffers as they quench the signal on the MALDI.  Avoid DMSO or glycerol if possible.  If not, keep percentage of glycerol/DMSO to <5%.
 
<em>Volume</em>: 1-10ul.  More is better.
 
<em>Containers</em>: Eppendorf type tubes, with your initials, sample name and date clearly on tube.

<strong>Samples for digest and protein identification:</strong>
<em>Amount</em>: Optimally, >500 fmol or >50 ng.  We can detect as low as 5 ng of protein but the coverage is not great, and the identification is less likely to work.  For post-translation modification searches, it is important to give at least 4-5 pmol of sample.
 
<em>Stain</em>: Coomasie, Sypro,  or other mass spec compatible silver stain.  Stay away from silver stain containing gluteraldehydes.  Do not over-stain gel.
 
<em>Controls</em>: Please provide blank portion of gel.  For your benefit provide a known, not pre-stained, standard.  Controls are not charged.
 
<em>Containers</em>: Place gel slice in methanol rinsed 1.5ml Eppendorf tube.(No need for additional liquid).  Clearly label all tubes with your initials, sample name and date.
<h4>Turnaround Time</h4>
<h4>Pricing</h4>[/three_fourth][one_fourth_last]
<div class="proteomics"><span class="amatic">How to...</span>
<h5>Place an Order</h5>
Login to iLab
<h5>Sample Drop-Off</h5>
We collect our samples from the following locations:
- The Fish Room (near the 3rd floor elevator lobby of the Smith Building)
- CLS Building (11th floor, near the Spiegelman Lab)
- <em>For RNA</em>: Smith Building, 1129 (-80C)
<h5>Get Data</h5>
<a href="http://mbcftp.dfci.harvard.edu/Login" target="_blank">MBCFTP</a>
<h5>Ask a Question</h5>
Send an <a href="mailto:zherbert@mail.dfci.harvard.edu" target="_blank">email to Zach</a></div>[/one_fourth_last]