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<h6>The Illumina MiSeq is a Next-Generation DNA sequencer with a single-laned flowcell that is capable of generating 15-25 million reads in one run. Sample libraries are loaded directly onto the MiSeq where amplified clusters are generated on the flowcell followed by sequencing by synthesis. There are several different sized reagent kits available, 50cycles, 150cycles, 300cycles, 500cycles, and 600cycles (1 cycle = 1bp). In addition, samples can be multiplexed or pooled and run together in the same lane as the MiSeq supports single and dual index reads up to 12bp in length.</h6>
<h4>Tech Specs</h4>
Standard Run Programs*:
1 X 50bp (SE50), 15M clusters
2 X 75bp (PE75), 25M clusters
2 X 30bp (PE30), 15M clusters
2 X 150bp (PE150), 15M clusters
2 X 250bp (PE250), 15M clusters
2 X 300bp (PE300), 25M clusters

*-Read lengths can be customized. For example, a 300cycle kit can be used for 1X300bp, 2X150bp, or 200bpX100bp.
<h4>Sample Requirements</h4>
<strong>Library Concentration:</strong> &gt;=10nM
<strong>Volume:</strong> &gt;=10ul
<strong>Quality Control:</strong> Performed by core staff.

Need Help? Check out our NGS library prep services
<h4>Turnaround</h4>
Data is generally available within 1 week.
<h4>Pricing</h4>
For detailed pricing and ordering please register or login to iLab[/three_fourth][one_fourth_last]
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<h5>Place an Order</h5>
Login to iLab
<h5>Sample Drop-Off</h5>
We collect our samples from the following locations:
- The Fish Room (near the 3rd floor elevator lobby of the Smith Building)
- CLS Building (11th floor, near the Spiegelman Lab)
- <em>For RNA</em>: Smith Building, 1129 (-80C)
<h5>Get Data</h5>
<a href="http://mbcftp.dfci.harvard.edu/Login" target="_blank">MBCFTP</a>
<h5>Ask a Question</h5>
Send an <a href="mailto:zherbert@mail.dfci.harvard.edu" target="_blank">email to Zach</a></div>
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